Friday, July 19, 2013

of course cancer is caused by bacteria..., told you so years ago

bytesizebio | Cancer and microbiology have been closely linked for over 100 years. Cancer patients are usually immunosuppressed due to chemotherapy, requiring special treatment and conditions to prevent bacterial infection. Bladder cancer is typically treated with inactivated tuberculosis bacteria to induce an inflammatory response which turns against remaining cancer cells, with remarkably effective results.  Also, viruses are known to cause cancer, including  papillomavirus (cervical cancer), Hepatitis B (liver cancer), and  HTLV (human T-lymphocyte virus, causing lymphoma). In 1982, the bacterium  Helicobacter pylori was discovered to be the main cause of gastric ulcers, and the first direct link between bacteria and cancer — stomach cancer — was established. The link between chronic ulcers and stomach cancer was already well known: what was not knows is that bacteria were the initial cause of stomach ulcers. Since then, several other suspects have been named, including links between Chlamydia and lung cancer, and  Salmonella and gallbladder cancer.
Inflammation changes the gut ecosystem 
There are two fields in which are not  generally thought of as being linked: microbial ecology and cancer research.  When we think of microbial ecology, we think of agricultural soil enrichment, marine ecology, air quality, nutrient recycling, species interaction,  diversity and all that jazz. Not of cancer though. But in the past five years we have amassed more genomic DNA data than we have in the 50 years preceding them, including data from cancer tissue and associated bacteria. These data are beginning to show us that that the links between microbial populations and cancer are more prevalent, complex and intimate than we thought. Bacteria, as microbiologists keep repeating ad nauseam, make up 90% of the cellular population of our bodies (the extra 10% are, well, us).  Following metagenomic sequencing, human microbial flora have been shown to affect conditions as varied as obesity, metabolic disease (including diabetes)  infant growth and colorectal cancer — all of which we have not associated with bacteria until recently. As a result the people who study bacteria, and the people who study cancer are working together more than ever before. Last year, a study in Science  led by a group from the University of North Carolina Chapel Hill, has shown a clear mechanistic link between microbial communities, inflammation, and colorectal cancer. In a nutshell, their study suggests that the following sequence of events takes place: 1) inflammation disturbs gut ecosystems; 2) this disturbance to conditions that allow pathogens to invade the gut; 3) the pathogens damage the host cells increasing the risk of the development of colorectal cancer. The study used mice that lacked the gene that makes Interleukin-10 (IL-10). IL-10 suppresses the inflammatory response, and IL10-deficient mice (IL10-/-) are genetically prone to gut inflammation.  The team compared bacterial communities in the inflamed guts of IL10-/- mice with those in healthy normal (“wild type”) mice. They found that the diversity of different kinds of bacteria was significantly lower in the IL10-/- mice. But the team found little difference in microbial diversity between mice that simply had inflammation and those that had inflammation and cancer, indicating that the inflammation was the critical factor affecting bacterial populations, reducing the diversity of bacteria in the colon. In fact, one major species to shoot up and dominate the inflamed gut was E. coli, another was Enterobacter faecalis. But IL10-/- mice that were inoculated with E. faecalis only rarely developed cancer, while 80% of the group with E. coli did. Specifically, E. coli strain NC101 was foind to be the culprit. The NC101 strain has a cluster of genes under the name of “pks island”. In 2010 a group from Toulouse found that pks island genes cause cellular replication and DNA damage in the host: the harbingers of cancer.  The UNC researchers colonized a mouse gut cell-line with E. coli that had the pks genes, and with E. coli lacking the pks genes. The inflammation remained, but the cells inoculated with E. coli without the pks developed fewer tumors. While mice take longer to develop tumors, the researchers saw 80% more DNA damaged cells in gut cells of IL10-/- mice inoculated with E. coli that had pks genes, versus those that were associated with E. coli without the pks genes.
Bacterial DNA in Cancer Cells
But the link between bacteria and cancer may run deeper than a changing microbial community. A recent study by a group at the University of Maryland School of Medicine shows that bacteria DNA gets transferred to human cells, in a process known as lateral gene transfer, or LGT.  LGT is known to occur quite commonly between bacteria, including bacteria of different species. In fact, that is how antibiotic resistance is transferred so quickly. But findings of bacterial LGT to humans are generally treated as possible experimental artifacts, rather than true events. The UMSM team scanned data from from the 1,000 Genomes Project and found more than 7,000 instances of LGT from bacteria to human cells. When they analyzed sequences from the Cancer Genome Atlas, they discovered 691,000 more cases  of LGT, and an overwhelming majority of LGT findings came from tumor samples, not from healthy cells. They found that DNA from Acinetobacter , was integrated into the genome of acute myeloid leukemia cells, especially in the mitochondrial genome. Acinetobacter is a soil bacterium but some species are known to be oppotunitic pathogens. For unknown reasons, this bacterium’s DNA was found more frequently in the genome of myeloid leukemia cells. Fist tap Dale.


CNu said...

Shades of "leeching and bloodletting"!!! What will the allopathic oncological barber-chirurgeons do with their multi-billion $$$ cancer poison/burn industry when it becomes common knowledge that cancer is caused by bacteria?

BigDonOne said...

CANCER -- What you have to do to cure it, after
you've got it, is not necessarily related to what caused it in the first place....
(cancer survivor)

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