DAYYUM! There Go Those Virus Reparations Lawsuits - Er, Uh, You See What Happened Wuz...,

washingtontimes |  Meanwhile, a number of suspicious actions and a paper trail suggest that the virus escaped from one of the labs, though China is clamping down on the ability to pursue those leads.

 “The most logical place to investigate the virus origin has been completely sealed off from outside inquiry by the CCP,” said the document, referring to the Chinese Communist Party.

The party has taken draconian steps to control information about the virus since January.

“A gag order to both places was issued on Jan. 1, 2020, and a major general from the PLA who is China’s top military microbiologist essentially took over the [Wuhan Institute of Virology] since mid-January.”

Labs face scrutiny

Both of the labs under scrutiny in the report have conducted extensive research on bat coronaviruses, including those that have close molecular similarities to SARS-Cov-2, the full designation of the new pathogen.

Among the most significant circumstantial evidence identified in the report are the activities of Shi Zhengli, a leader in bat coronavirus research with the Wuhan Institute of Virology, China’s only high-security, level four research laboratory.

Ms. Shi has been involved in bioengineering bat coronaviruses, and a medical doctor named Wu Xiaohua launched an online campaign to expose Ms. Shi’s work. Dr. Wu said she believes the coronavirus at the root of the pandemic is one of 50 viruses in a database Ms. Shi manages.

The document also points to a 2015 academic report in Nature Medicine by Ms. Shi and 14 other scientists who said that while researching the potential for bat coronaviruses to infect humans, “we built a chimeric virus encoding a novel, zoonotic [animal-origin] spike protein … that was isolated from Chinese horseshoe bats.”

The scientists said the “hybrid virus” allowed researchers to study the ability of the virus to “cause disease.”

Dr. Wu stated in an internet posting that Ms. Shi used laboratory animals to test the human-infecting virus, and one of those animals may have been the origin of the pandemic.

Dr. Wu also asserted that the institute’s virus-carrying animals had been sold as pets, dead laboratory animals were not properly disposed of, and lab workers were known to boil and eat laboratory-used eggs.

“Wu’s charges of WIV management negligence are specific and have not been convincingly rebutted by WIV,” the analysis said.

Ms. Shi has worked closely with several U.S. virologists, and some American scientists have defended her and the institute from critics who point to her work with bat viruses as a needed focus of an investigation, the analysis says. Ms. Shi, in response to Dr. Wu’s assertions, said in March on her social media account: “I promise with my life that the virus has nothing to do with the lab.”

We Made The Coronavirus Epidemic: NYTimes Called Out Shi Zhengli A Month Ago

NYTimes |  Despite the new virus’s name, though, and as the people who christened it well know, nCoV-2019 isn’t as novel as you might think.

Something very much like it was found several years ago in a cave in Yunnan, a province roughly a thousand miles southwest of Wuhan, by a team of perspicacious researchers, who noted its existence with concern. The fast spread of nCoV-2019 — more than 4,500 confirmed cases, including at least 106 deaths, as of Tuesday morning, and the figures will have risen by the time you read this — is startling but not unforeseeable. That the virus emerged from a nonhuman animal, probably a bat, and possibly after passing through another creature, may seem spooky, yet it is utterly unsurprising to scientists who study these things.

One such scientist is Zheng-Li Shi, of the Wuhan Institute of Virology, a senior author of the draft paper (not yet peer reviewed and so far available only in preprint) that gave nCoV-2019 its identity and name. It was Ms. Shi and her collaborators who, back in 2005, showed that the SARS pathogen was a bat virus that had spilled over into people. Ms. Shi and colleagues have been tracing coronaviruses in bats since then, warning that some of them are uniquely suited to cause human pandemics.

In a 2017 paper, they set out how, after nearly five years of collecting fecal samples from bats in the Yunnan cave, they had found coronaviruses in multiple individuals of four different species of bats, including one called the intermediate horseshoe bat, because of the half-oval flap of skin protruding like a saucer around its nostrils. The genome of that virus, Ms. Shi and her colleagues have now announced, is 96 percent identical to the Wuhan virus that has recently been found in humans. And those two constitute a pair distinct from all other known coronaviruses, including the one that causes SARS. In this sense, nCoV-2019 is novel — and possibly even more dangerous to humans than the other coronaviruses.

what is entosis?

TheScientist | Though the mechanisms of engulfment may differ among cell and cancer types, the end result is nearly indistinguishable—a whole, living cell is housed within a large vacuole inside a tumor cell. Internalized cells usually follow one of three paths. They can continue living, at least temporarily, within the host cell, even dividing within their vacuole homes. Occasionally, they escape from the host cell to once again become a single, individual cell in the extracellular space. By and large, however, death is the most common fate for cells engulfed by tumor cells. Wang and colleagues demonstrated evidence of the apoptotic death of NK cells following their uptake by tumor cells. Nearly 90 percent of the internalized lymphocytes underwent traditional, programmed cell death, as evidenced by the activation of caspase 3. Work by Overholtzer’s group, on the other hand, suggests that cell engulfment between tumor cells represents a different type of cell death altogether—one mediated by lysosomes. The vacuoles housing the internalized tumor cells, his group observed, became acidic and surrounded by lysosomal membranes, indicative of fusion with lysosomes. Furthermore, when the researchers overexpressed B-cell lymphoma 2 (Bcl-2) to inhibit apoptosis, it had little effect on the death of internalized cells. On the other hand, inhibiting lysosomal acidification of the vacuoles could rescue the captured cells, when it was combined with apoptotic inhibitors. Interestingly, when only the lysosomal inhibitors were introduced, more cells appeared to undergo an apoptotic death, suggesting that apoptosis serves as a backup mechanism to the more common lysosomal death of the internalized cells.

Fais suggests it is simply the acidic environment of the tumor-cell vacuoles in metastatic melanoma cells that kills the internalized lymphocytes, though lytic enzymes may help to further digest the cell, he says. He argues that the engulfment and subsequent killing of cells such as lymphocytes is cell cannibalism in the most literal sense—one cell eating another. Once the victim is digested, the tumor cell can theoretically derive nutrients from it, promoting cancer survival and growth.

“We know that nutritional stress is a common feature of tumors,” says Eileen White, a cancer biologist at The Cancer Institute of New Jersey and Rutgers University. “We know they’ll undergo this process of autophagy where they’ll eat themselves. If they have the capability of eating each other or other cells—that would open a whole new door for tumors to sustain themselves.”

As evidence for this hypothesis, Fais showed in vitro that cell cannibalism increased under starvation conditions, and that the ingestion of T cells promoted the survival of melanoma cells. “The T cell is great because it has all these wonderful complex carbohydrates on the surface,” says cancer biologist Thomas Seyfried of Boston College. “They can all be degraded to glucose and other fuels [that tumor cells] could be using.”

But even if cells are deriving nourishment from their cannibalistic activities, it’s likely not the only benefit of the behavior, says immunologist Yufang Shi, who studies apoptosis at the Chinese Academy of Sciences and the Child Health Institute of New Jersey. “For one cell to digest another cell and to get energy . . . this is very uneconomical,” Shi explains. “You have to really make the cell into amino acids and polysaccharides. It’s very hard to use that as energy.” The fact that cell cannibalism increased when the cells were starving may simply be due to the fact that nutrient deprivation can cause cells to become detached from the extracellular matrix, Shi added—an event that Overholtzer’s group suggests could promote cell engulfment as a result of imbalanced cell-cell adhesion forces.

Another possibility is that the engulfed cells are driving the process. Internalized immune cells, for example, may have the potential to suppress tumor growth. During his initial graduate studies in the 1980s and again when he resumed this work more recently, Wang observed that some NK cells internalized by tumor cells can actually kill their host cells from the inside out. “After they enter into the tumor cells, they make the tumor cells erupt,” Wang says. “When [these NK cells] die, they also release a lot of enzymes,” Shi explains. “They are cytotoxic cells, so they can kill by releasing directly into the target cell, like the suicide bombers.”

But whether the internalized NK cells are initiating the engulfment is still unclear. If, on the other hand, the tumor cells are actively consuming the lymphocytes, it could provide a way for cancer to evade attack by the immune system. “I have a suspicion that maybe tumor [cells] in some conditions can kill the NK cells as a way to escape the surveillance of the immune system,” Wang says. This may become particularly important as the cancer metastasizes, Yao adds. “One of the physical challenges for those tumor cells will be how to survive in the new sites. One way is by taking [up] those NK cells and other immune cells to damage the immune response of cancer [patients].”

The bizarre phenomenon may also contribute to the genetic instability of cells, perhaps contributing to the formation of cancer early on. This March, Overholtzer and colleagues published the finding that cell-in-cell structures can act as cleavage barriers that disrupt cell division, leading to changes in ploidy—the number of sets of chromosomes in the cell—which are known to drive tumor progression.5 Conversely, cell engulfment may act to suppress tumor growth, such as when tumor cells eat other tumor cells. “Entosis has a dual nature,” says Overholtzer. “It clearly can kill [tumor] cells, but also, it can disrupt ploidy—one is predicted to be tumor suppressive, one is tumor promoting.”

For now, the question of function remains “a puzzle,” Fais says, and “I don’t have all the pieces.” But with evidence growing for significance of cell engulfment in tumor pathogenesis, researchers are now considering whether the phenomenon could serve to aid in diagnosis or in the development of new cancer treatments. “I think in the next few years this will be a very active field,” Shi says.

The Bulletin Of The Atomic Scientists DESTROYS Any Further Doubt About Covid's Origin

 Remember Where You Read It First!!! 

This is School For You!!!

Accept No MuthaPhukkin Substitutes...,

thebulletin |  Why would anyone want to create a novel virus capable of causing a pandemic? Ever since virologists gained the tools for manipulating a virus’s genes, they have argued they could get ahead of a potential pandemic by exploring how close a given animal virus might be to making the jump to humans. And that justified lab experiments in enhancing the ability of dangerous animal viruses to infect people, virologists asserted.

With this rationale, they have recreated the 1918 flu virus, shown how the almost extinct polio virus can be synthesized from its published DNA sequence, and introduced a smallpox gene into a related virus.

These enhancements of viral capabilities are known blandly as gain-of-function experiments. With coronaviruses, there was particular interest in the spike proteins, which jut out all around the spherical surface of the virus and pretty much determine which species of animal it will target. In 2000 Dutch researchers, for instance, earned the gratitude of rodents everywhere by genetically engineering the spike protein of a mouse coronavirus so that it would attack only cats.

Virologists started studying bat coronaviruses in earnest after these turned out to be the source of both the SARS1 and MERS epidemics. In particular, researchers wanted to understand what changes needed to occur in a bat virus’s spike proteins before it could infect people.

Researchers at the Wuhan Institute of Virology, led by China’s leading expert on bat viruses, Shi Zheng-li or “Bat Lady,” mounted frequent expeditions to the bat-infested caves of Yunnan in southern China and collected around a hundred different bat coronaviruses.

Shi then teamed up with Ralph S. Baric, an eminent coronavirus researcher at the University of North Carolina. Their work focused on enhancing the ability of bat viruses to attack humans so as to “examine the emergence potential (that is, the potential to infect humans) of circulating bat CoVs [coronaviruses].” In pursuit of this aim, in November 2015 they created a novel virus by taking the backbone of the SARS1 virus and replacing its spike protein with one from a bat virus (known as SHC014-CoV). This manufactured virus was able to infect the cells of the human airway, at least when tested against a lab culture of such cells.

The SHC014-CoV/SARS1 virus is known as a chimera because its genome contains genetic material from two strains of virus. If the SARS2 virus were to have been cooked up in Shi’s lab, then its direct prototype would have been the SHC014-CoV/SARS1 chimera, the potential danger of which concerned many observers and prompted intense discussion.

“If the virus escaped, nobody could predict the trajectory,” said Simon Wain-Hobson, a virologist at the Pasteur Institute in Paris.

Baric and Shi referred to the obvious risks in their paper but argued they should be weighed against the benefit of foreshadowing future spillovers. Scientific review panels, they wrote, “may deem similar studies building chimeric viruses based on circulating strains too risky to pursue.” Given various restrictions being placed on gain-of function (GOF) research, matters had arrived in their view at “a crossroads of GOF research concerns; the potential to prepare for and mitigate future outbreaks must be weighed against the risk of creating more dangerous pathogens. In developing policies moving forward, it is important to consider the value of the data generated by these studies and whether these types of chimeric virus studies warrant further investigation versus the inherent risks involved.”

That statement was made in 2015. From the hindsight of 2021, one can say that the value of gain-of-function studies in preventing the SARS2 epidemic was zero. The risk was catastrophic, if indeed the SARS2 virus was generated in a gain-of-function experiment.

WaPo Throwing Shade On Shi Zhengli And The Wuhan Virological Institute

WaPo |  Two years before the novel coronavirus pandemic upended the world, U.S. Embassy officials visited a Chinese research facility in the city of Wuhan several times and sent two official warnings back to Washington about inadequate safety at the lab, which was conducting risky studies on coronaviruses from bats. The cables have fueled discussions inside the U.S. government about whether this or another Wuhan lab was the source of the virus — even though conclusive proof has yet to emerge.

In January 2018, the U.S. Embassy in Beijing took the unusual step of repeatedly sending U.S. science diplomats to the Wuhan Institute of Virology (WIV), which had in 2015 become China’s first laboratory to achieve the highest level of international bioresearch safety (known as BSL-4). WIV issued a news release in English about the last of these visits, which occurred on March 27, 2018. The U.S. delegation was led by Jamison Fouss, the consul general in Wuhan, and Rick Switzer, the embassy’s counselor of environment, science, technology and health. Last week, WIV erased that statement from its website, though it remains archived on the Internet.

What the U.S. officials learned during their visits concerned them so much that they dispatched two diplomatic cables categorized as Sensitive But Unclassified back to Washington. The cables warned about safety and management weaknesses at the WIV lab and proposed more attention and help. The first cable, which I obtained, also warns that the lab’s work on bat coronaviruses and their potential human transmission represented a risk of a new SARS-like pandemic.

“During interactions with scientists at the WIV laboratory, they noted the new lab has a serious shortage of appropriately trained technicians and investigators needed to safely operate this high-containment laboratory,” states the Jan. 19, 2018, cable, which was drafted by two officials from the embassy’s environment, science and health sections who met with the WIV scientists. (The State Department declined to comment on this and other details of the story.)

The Chinese researchers at WIV were receiving assistance from the Galveston National Laboratory at the University of Texas Medical Branch and other U.S. organizations, but the Chinese requested additional help. The cables argued that the United States should give the Wuhan lab further support, mainly because its research on bat coronaviruses was important but also dangerous.

As the cable noted, the U.S. visitors met with Shi Zhengli, the head of the research project, who had been publishing studies related to bat coronaviruses for many years. In November 2017, just before the U.S. officials’ visit, Shi’s team had published research showing that horseshoe bats they had collected from a cave in Yunnan province were very likely from the same bat population that spawned the SARS coronavirus in 2003.

Controlaspecies: Gain Of Function Research And The Blame Game

harvardtothebighouse |  we have Peter Daszak.  His company, EcoHealth Alliance, which is a non-profit that depends largely on multi-million dollar government grants to function, has been partnering with Chinese researcher for years in an attempt to secure funding for more and more research into coronaviruses. At least they’re not really even pretending to be philanthropic.

And in one of the more transparent attempts at blatant PR-spin, Daszak was featured alongside one of the researchers who learned how to create hyper-virulent bat coronaviruses at UNC back in 2015, Zhengli Shi. Their article insists we should take Zhengli at her word when she claims to have not found a match after she checked COVID-19’s genome against everything in her lab. As if someone responsible for releasing the most virulent pathogen to hit humanity in modern history, one that’s already killed thousands and is projected to kill millions and millions more all across the globe, would simply fess-up to it, torpedoing her career and the years of research performed by her and her colleagues? And possibly opening all of them up to legal and other repercussions?

If you still aren’t sure whether the scientists involved with kind of research are being forthright, there’s Dr. Ralph Baric. It was in his lab at UNC that a hyper-virulent bat Franken-virus was created by splicing a new protein-spike on an existing coronavirus, creating a monster so vicious that a virologist with the Louis Pasteur Institute of Paris warned: “If the [new] virus escaped, nobody could predict the trajectory.” It should also be noted that several years prior to tinkering directly with bat coronavirus spike-proteins, Baric orchestrated research that involved isolating a coronavirus from civets and then passing it through mammalian ACE2 receptor cells that were grown in the lab from kidney and brain samples – serial passage through host cell lines instead of entire hosts, which imparted a strong affinity for ACE2, and presumably created an airborne strain of coronavirus. And if cells derived from kidneys and brains were used for the serial passage development of COVID-19, that might help explain its affinity for attacking the kidneys and brains of its human hosts.

So if he was being honest, you might expect him to warn the public about the lethal potential coronaviruses pose during our current outbreak. However, when he was asked if the public should be worried about COVID-19 he said that people should be more worried about the seasonal flu. Pretty bizarre statement from a scientist who knew full well how dangerous coronaviruses could be, especially given the fact that not only was Zhengli Shi working in his lab on that project in 2015, but Xing-Yi Ge was too. Both of whom returned to Wuhan where they’ve continued their work for years.

Xing-Yi Ge is especially notable since in 2013 he became the very first scientist to isolate a bat coronavirus from nature that uses the ACE2 receptor, which is found in human, tree shrew, and ferret lungs and allows coronaviruses to become airborne. And as you might have learned by now, that’s the exact receptor used by COVID-19 to enter human cells – if anyone would know how to finagle that part of the coronavirus genome, it’d be him. So both Xing-Yi Ge and Zhengli Shi were part of the research team that created this hybridized hyper-virulent bat coronavirus under Baric, who’s actively downplayed the risk posed by COVID-19, and then returned to work in Wuhan, where funding provided in part by Daszak’s company allowed them to continue their work on coronaviruses with plenty of research to cut-and-paste into their work at the Wuhan Institute of Virology’s Disease Engineering Technical Research Center.

And as Dr. Ian Malcolm puts it in Jurassic Park, it is never a good idea to futz around with science and research when you don’t fully understand it, nor its possible implications.

However it wasn’t just Daszak funding their work, Zhengli also secured millions of dollars in grant money from various American institutions including our Department of Defense as well as the U.S. Biological Defense Research Directorate, and millions more from other foreign governments.

So although the Chinese Communist Party deserves its share of the blame for attempting to cover the outbreak up, arresting the heroic scientists trying to warn us and issuing gag-orders and the destruction of evidence, this research likely wouldn’t have occurred at all if the NIH hadn’t lifted the ban on gain-of-function research in the first place. And it was funded directly by American tax dollars, by government officials willing to let others play god at their behest.

But now that the virus is out of the lab, are the private entities responsible for its creation going to bear any of the blame at all? Or will America and China continue to point fingers at each other until the worst happens?

“Mars will accuse Earth of using a bio-weapon. Earth will claim it was Mars. The Belt will blame the other two. It’s a good way to start a war and cover it up.”

One last spoiler warning… okay, so in The Expanse the central plot device pushing things forward is the discovery of a mysterious substance dubbed the protomolecule, which seems to have a mind of its own and seek out radiation as sustenance before then beginning “the Work,” a mysterious intergalactic goal that isn’t revealed until later seasons.

And its not individual nations who first attempt to harness the protomolecule, but their Peter Daszak, the aforementioned scientist and CEO named Jules-Pierre Mao, who attempts to weave it into the genomes of immuno-compromised children to create hybridized super-soldiers. Not for his own private army, but as a game-changing bio-weapon he’ll sell to whichever government is willing to pay the most for it. So in The Expanse, it takes amoral scientists as well as the collusion of officials affiliated with both governments for this research to happen and be hidden, and when these Hybrids are eventually dropped between both armies the carnage is immense.

Luckily, we haven’t gotten that far on earth yet, but the rhetoric between America and China has been heading in that direction – it’s been growing increasingly hostile as each blames the other for starting the pandemic and covering it up, with China even going so far as to threaten to cut off our supply of antibiotics and other life-saving medical goods.  Meanwhile Daszak, Baric, Zhengli, and others sit back counting their lucky stars and their money, since both governments and the public at large seem to have bought their story that there’s no way this virus leaked out of one of their labs, and every government on earth now wants to harness their research to help create vaccines and treatments.

And these researchers have been assisted by scientifically spurious and journalistically vacuous articles which mindlessly regurgitate claims from the Chinese government, and its scientific propaganda arm, the WHO, about how bad the outbreak was in the past and how contained it is now. As the Chinese government arrested whistle-blowers and sent agents out into the street in bio-hazard gear while carrying automatic weapons to detain anyone suspected of breaking quarantine, while literally welding apartments buildings shut, the American media fawned over China’s “decisive and heroic” actions.

Please take a moment to consider the fact that almost everyone reading the news to you on television was selected due to their connections or how photogenic they are, not because of any actual journalistic chops or ability to think critically.

So as two superpowers are pushed closer and closer to conflict, the research that’s almost certainly the source of COVID-19 not only continues unabated, but if anything talk of more funding to stop this sort of supposedly natural pandemic from happening again is pouring into the pockets of the people who, if they weren’t directly responsible, should certainly have been at the forefront of warning the world about the risks posed by lab-altered coronaviruses, and been disclosing the existence of this sort of research in the first place.

Oddly, each and everyone one of them is pretending that viral dual-use gain-of-function research has never occurred at all. Or not so oddly, when you stop and think about how much they have to lose if their role in this pandemic is revealed.

“The hardest part of this game is figuring out who the enemy really is.”

Other than the fact it doesn’t bear the direct marks of genetic tampering, just like the engineered hyper-virulent H5N1 Bird Flu, there’s literally nothing natural about COVID-19’s behavior or clinical presentation. And hauntingly, peer-reviewed research has noted that a crucial region of its genome “may provide a gain-of-function… for efficient spreading in the human population.”

Not only is it so distant from any other coronavirus that it forms its own clade, but there isn’t even a natural path for it to have emerged through – assertions about pangolins have always been dubious at best, but were even further debunked when analysis of COVID-19’s genome at the regions that most accurately show heritage made it “very unlikely” that pangolins had ever been involved at all.

Beyond that is the fact that its affinity for the ACE2 receptor is somewhere between 10 and 20 times higher than SARS, and it also creates viral loads thousands of times higher than SARS. These two characteristics point towards COVID-19 using antibody-dependent enhancement, or ADE, to enter human cells. This is when the virus is able to hijack white blood cells to more easily enter into the rest of our body’s cells, allowing it to seep deep into its hosts’ nervous systems, creating permanent neurological damage in the hosts it doesn’t kill outright. ADE could also explain why between 5% and 10% of once “recovered” patients in Wuhan have been showing up with fresh infections, since that phenomenon allows a virus to hijack the antibodies created by a previous infection to re-attack an old host. And curiously Zhengli Shi, of UNC and Wuhan fame, co-authored a 2019 paper which used inert viral shells to figure out exactly how SARS, with its affinity to the ACE2 receptor just like COVID-19, was able to harness ADE to hijack white blood cells for enhanced cell entry. A gain-of-function extension of this research would be exactly the kind of experiment that could’ve given birth to COVID-19, especially considering that 2019 paper managed to fine-tune the exact concentration of antibodies that would best facilitate ADE.

Both HIV and Dengue Fever use antibody-dependent enhancement to boost their virulence, however its generally a phenomenon that takes a long time to occur when it happens in nature. However COVID-19 looks like it may have had its ADE jacked into hyper-drive as it was passed between a series of animal hosts, since it has the aforementioned much stronger ability to bind to host cells and creates viral loads orders of magnitude higher, and also appears to immediately to be able to enter its hosts nervous systems, killing many of its victims by attacking the region of the brain that controls breathing, drastically lowering white blood cell counts early on in infections, and apparently re-infecting individuals who had already appeared to clear their infection.

Further increasing the possibility that COVID-19’s unique clinical presentation may be due to its ADE being juiced by laboratory engineering are the observations from an ER doctor who’s stated that I have seen things that I have never seen before… I have witnessed medical phenomenon that just don’t make sense in the context of treating a disease that is supposed to be viral pneumonia. In an interview with Medscape, Dr. Cameron Kyle-Sidell went on to say that the closest thing to the symptoms he was witnessing in his emergency room.

Remember Where You Heard It First (Last March-April)

 

Seriously - Am I The Only Cat Fixated On This LOW HANGING SARS-CoV2 FRUIT?!?!?!

thescientist |  Ralph Baric, an infectious-disease researcher at the University of North Carolina at Chapel Hill, last week (November 9) published a study on his team’s efforts to engineer a virus with the surface protein of the SHC014 coronavirus, found in horseshoe bats in China, and the backbone of one that causes human-like severe acute respiratory syndrome (SARS) in mice. The hybrid virus could infect human airway cells and caused disease in mice, according to the team’s results, which were published in Nature Medicine.

The results demonstrate the ability of the SHC014 surface protein to bind and infect human cells, validating concerns that this virus—or other coronaviruses found in bat species—may be capable of making the leap to people without first evolving in an intermediate host, Nature reported. They also reignite a debate about whether that information justifies the risk of such work, known as gain-of-function research. “If the [new] virus escaped, nobody could predict the trajectory,” Simon Wain-Hobson, a virologist at the Pasteur Institute in Paris, told Nature.

In October 2013, the US government put a stop to all federal funding for gain-of-function studies, with particular concern rising about influenza, SARS, and Middle East respiratory syndrome (MERS). “NIH [National Institutes of Health] has funded such studies because they help define the fundamental nature of human-pathogen interactions, enable the assessment of the pandemic potential of emerging infectious agents, and inform public health and preparedness efforts,” NIH Director Francis Collins said in a statement at the time. “These studies, however, also entail biosafety and biosecurity risks, which need to be understood better.”

Baric’s study on the SHC014-chimeric coronavirus began before the moratorium was announced, and the NIH allowed it to proceed during a review process, which eventually led to the conclusion that the work did not fall under the new restrictions, Baric told Nature. But some researchers, like Wain-Hobson, disagree with that decision.

The debate comes down to how informative the results are. “The only impact of this work is the creation, in a lab, of a new, non-natural risk,” Richard Ebright, a molecular biologist and biodefence expert at Rutgers University, told Nature.

But Baric and others argued the study’s importance. “[The results] move this virus from a candidate emerging pathogen to a clear and present danger,” Peter Daszak, president of the EcoHealth Alliance, which samples viruses from animals and people in emerging-diseases hotspots across the globe, told Nature.

Wuhan Institute of Virology: HATERS HURT'N MY FEELINGS! I DINDU NUFFIN!!!

SCMP |  A Chinese research institute in the city of Wuhan – ground zero of the coronavirus outbreak

– has again dismissed rumours suggesting it is linked to the public health crisis, saying it has been “badly hurt” by conspiracy theories circulating online.

 

“The rumours … have caused severe damage to our researchers who have been dedicated to working on the front line, and seriously interrupted the emergency research we are doing during the epidemic,” the Wuhan Institute of Virology (WIV), which is affiliated with the Chinese Academy of Sciences, said in a statement.

Those rumours included that the new virus strain was “man-made”, “leaked from the WIV lab”, that “the WIV was taken over by the military”, “a WIV researcher died from the leaked virus”, “a WIV student is patient zero”, and “a WIV researcher reported to authorities that the WIV chief was responsible” for the epidemic, the statement posted on its website on Wednesday said.

The institute runs the Wuhan National Biosafety Laboratory, the only facility in China equipped to diagnose and research easily transmitted pathogens at the highest biosafety level of four.

“Looking back on our hard work over the past month, we have nothing to be ashamed of or to regret,” the statement said.

A prominent virologist with the institute has also been targeted by the rumours. Shi Zhengli’s exploration of caves in Yunnan province discovered that another deadly coronavirus – which caused the severe acute respiratory syndrome, or Sars, epidemic in 2002-03 – had originated in bats. Her database of viruses found in bats provided evidence for the theory that the coronavirus at the centre of the ongoing epidemic was also linked to bats. The new virus strain was found to be 96 per cent identical to one found in bats.

---------------------------------------------------------------------------------------------------------------------------

 

I'm not a virologist and don't pretend to be. But I can read a fugging manual with the best of them, and understand what I've read. That said, slowly reread the nature paper from 2015 yourself. 

Therefore, to examine the emergence potential (that is, the potential to infect humans) of circulating bat CoVs, we built a chimeric virus encoding a novel, zoonotic CoV spike protein—from the RsSHC014-CoV sequence that was isolated from Chinese horseshoe bats¹—in the context of the SARS-CoV mouse-adapted backbone. The hybrid virus allowed us to evaluate the ability of the novel spike protein to cause disease independently of other necessary adaptive mutations in its natural backbone. Using this approach, we characterized CoV infection mediated by the SHC014 spike protein in primary human airway cells and in vivo, and tested the efficacy of available immune therapeutics against SHC014-CoV. Together, the strategy translates metagenomics data to help predict and prepare for future emergent viruses.

Shi Zhengli - you know you done f'd up....,

 

I'm NOT The Only Cat Seriously Fixated On The Low-Hanging Cornucopia Of SARS-CoV2 Fruit

harvardtothebighouse |  Some of the dystopian carnage creeping across China may be due to the fact that much of China’s population may have already been exposed to coronavirus infection via SARS or other less notorious strains, which would allow the Wuhan Stain COVID-19 to use antibody-dependent enhancement to much more efficiently enter into cells, and then become much more virulent since this enhancement hijacks the body’s preexisting immune response to coronavirus infections and allows easier entry. However whether or not people have been exposed to a coronavirus infection before, once it’s been circulating in a population for long enough the Wuhan Strain may be able to reinfect its own past hosts and use this molecular hijacking on antibodies left from its own previous infection to become far more virulent, regardless of whether or not someone has been exposed to other coronaviruses before COVID-19. And early reporting from Chinese doctors indicates that re-infections of the Wuhan Strain are far more lethal than the first.

– Additionally, although another since-retracted pre-print noted several very short genomic sequences in COVID-19’s spike-protein gene that look far more similar to sequences found in HIV than to other coronaviruses – critics quickly pointed out that the shared homology didn’t reach statistical significance. However a closer look at the data reveals that there were a few small shared genomic segments that, despite being physically separated from each other along each strand of DNA, all worked together to code for the Wuhan Strain’s protein-spike’s crucial receptor binding site. Something that is highly unlikely to have happened by chance. And despite most of its protein-spike being shared with SARS, these substituted segments weren’t shared at all – nor were they found in any other coronavirus. One possible but likely reason for these HIV-like segments is that they were meant to be epitopes, or molecular flags meant to mark intruders for a vaccine to target. It is mathematically possible for this to happen in nature – but only in a ten-thousand bats chained to ten-thousand Petri dishes and given until infinity sense. Alternatively, it could also be produced by infecting a room full of ferrets with a bespoke coronavirus vaccine and sifting through the wreckage for your genomic needle.

– Critics have brushed off the Wuhan Strain’s shared homology with HIV as statistically insignificant, however clinical reporting indicates that the Wuhan Strain may be using this shared HIV homology to attack CD4 immune cells just like HIV does, as an unusually high percentage of patients are showing low white blood cell counts, especially the sickest ones. This pathogenicity may well be due to the unique HIV-live genomics of the Wuhan Strain, as one white-paper by LSU’s professor emeritus of Microbiology, Immunology, and Parasitology who’s also a Harvard-educated virologist with a PhD in Microbiology and Molecular Genetics notes: “This is the first description of a possible immunosuppressive domain in coronaviruses… The three key [mutations] common to the known immunosuppressive domains are also in common with the sequence from [the spike-protein]. While coronaviruses are not known for general immunosuppression of the style shown by HIV-1, this does not rule out immunosuppression at the site of active infection in the lung, which would prolong and potentially worsen infection at that site.” And early research has indicated that this unique region may make COVID-19 up to 1,000 times more likely to bind to human cells than SARS.

– Even more troubling, a peer-reviewed study noted that one particular part of the Wuhan Strain’s spike-protein genome also wasn’t found in any of its relatives, “and may provide a gain-of-function to [COVID-19] for efficient spreading in the human population.”  And according to that paper, this particular type of furin cleavage site makes similar viruses both more pathogenic and more neurotoxic.

– Evidence for the Wuhan Strain’s neurotoxicity arrived in late February, in a published paper which notes that “the most characteristic symptom of COVID‐19 patients is respiratory distress, and most of the patients admitted to the intensive care could not breathe spontaneously.” Combined with the observation that “some COVID‐19 patients also showed neurologic signs such as headache, nausea and vomiting,” this paper asserts that since SARS was found heavily concentrated in the brainstems of its autopsied victims, COVID-19 is also probably crossing the blood-brain barrier and killing its victims not just via pneumonia, but also by causing neurological respiratory failure.

– And it should be noted that SARS – much ballyhooed as a close relative to the Wuhan Strain – didn’t notable effect white blood cell counts.  Additionally, clinical treatment guides published online in late January by established Chinese medical sources note the progressive reduction of white blood cells, as well as the importance of monitoring this decline. And reporting from Thailand indicates that adding a cocktail of two different anti-HIV drugs to the typical flu treatment regime seemed to effectively knock back the Wuhan Strain. Additionally, one of the only autopsies performed outside of China to date found that the deceased had a severely depleted white blood cell count. These lowered counts may come from this shared similarity with HIV, or it could also be the result of antibody-dependent enhancement as well, since this phenomenon primarily targets white blood cells for its hijackings and may help explain why consecutive infections are so lethal.

– In a highly concerning turn, scientists have noted that the Wuhan Strain can have a “striking” short term rate of mutation which doesn’t indicate an artificial origin but captures the unique threat posed by this coronavirus regardless of its providence, since a faster mutation rates makes it more likely this virus can dodge testing and neutralize vaccines. Something there is already early evidence for. Further concerning are reports out of China that even patients who appear cured still harbor COVID-19 in their system, and although the full implications of this are not yet known – none of them are good.

– One of the worst possible scenarios for COVID-19’s mutation rate would be if it falls into the Goldilocks range that would allow it to form mutant viral swarms: too many mutations will cause a virus to eventually implode, not enough allows host immune systems to catch-up, but if things are just right mutant swarms can form and spread across host populations, burrowing into host nervous systems and causing permanent neurological damage. Mutant swarms form when a virus produces mutationally-damaged copies of itself inside a host, some of which aren’t infectious but find their way into the nervous system where they burrow in causing damage, and others that combine with complimentary broken copies inside host cells to produce working infectious copies of the virus. So a host can not only become crippled with neurological issues, but also still be producing infectious copies of the virus. And it seems as if COVID-19 has many characteristics that indicate the potential to form mutant swarms: the “striking” mutation rate mentioned above and the fact a second widespread mutated strain seems to have already emerged in Washington State with many other isolated strains reported elsewhere, crossing between species is another factor and a dog in Hong Kong appears to have tested positive, the fact that the Wuhan Strain can infect not only the respiratory tract but feces as well – multi-organ involvement is an important contributor to viral swarms, and finally the markedly viral load rate of COVID-19 compared to SARS – SARS produced a viral load several times lower which decreased over time, while COVID-19 produces a “very high” viral load that appears to increase over time and can peak several orders of magnitude higher than SARS was measured to reach. And alarming evidence that this phenomenon is occurring emerged from a Chinese pre-print which noted that over one-third of the roughly 200 patients studied has some neurological symptoms, with nearly half of the most severe patients exhibiting neurological issues.

– Another exceptional trait of the Wuhan Strain COVID-19 is that not only does it form its own clade, it’s calculated to have diverged from SARS and its other sister coronaviruses some 260 years ago. And yet in all that time, while it every other branch of the coronavirus tree was busy branching-off into countless variants, if it emerged naturally, COVID-19 somehow spent a quarter of a millennium as the lone known example of its clade, somehow not mutating into related lineages in all that time. Another simpler explanation is that this apparent hereditary distance and genetic uniqueness is the just the result of being altered in a lab. And although two distinct strains of COVID-19 have been identified, there’s no reason to believe this mutational differentiation happened before contact with humans in December of 2019. Additionally, when neutral sites, the specific points in the genome which most reliably show evolutionary change, were examined: COVID-19 looks even more evolutionarily distant from any of its possible relatives.

– Also giving credence to the idea that the Wuhan Strain was bio-engineered is the existence of a patent application registered to a scientist from Wuhan that looks to modulate a coronavirus’ spike-protein genes – the precise region altered by Zhengli Shi at UNC to make a hyper-virulent strain of coronavirus, and whose alteration and adaptation would explain the Wuhan Strain’s unusual behavior as discussed above.

russia and iran mull their syria options...,

rferl | U.S.-led military intervention in Syria would put Washington on a collision course with two unwavering allies of Syrian President Bashar al-Assad -- Iran and Russia.

Just how Tehran and Moscow might react is a key part of the calculus that U.S. President Barack Obama must consider in weighing his course of action in Syria.

Although analysts agree that neither country is likely to respond with direct military support for Assad, they also don't expect Tehran or Moscow to sit back passively. Iran's Islamic Revolutionary Guards Corps (IRGC) has said that an attack against Assad is a "red line" that would trigger a response, although it has not said what that response might be.

Iran's reaction to date has been mild, with Tehran condemning both the use of chemical weapons and threats of foreign military intervention.

According to Will Fulton, an Iran analyst with the American Enterprise Institute in Washington, the IRGC would likely not risk a direct confrontation with the United States but could act through proxies, including Hizballah in Lebanon or Shi'ite militias in Iraq.

Tehran would also seek to capitalize on anti-U.S. reaction at home and across the region. "I think we will absolutely see more condemnations, more warnings from IRGC and hard-line officials, and this will of course play into, especially, the IRGC's narrative that the conflict in Syria is a conspiracy of Israel and the West," Fulton says. "So they will use this attack to fuel that narrative and it will become a recruiting tool and a narrative defense of their own foreign interference in Syria."

J.K. Rowling: I Wish A N*gga Would Try That Pre-Crime Shi-ite On Me

MSN  |  K Rowling has challenged Scotland’s police to arrest her under the SNP’s new hate crime law after stating that a series of high-profile trans women are men.

The Harry Potter author, who lives in Edinburgh, wrote on X, formerly Twitter: “Freedom of speech and belief are at an end in Scotland if the accurate description of biological sex is deemed criminal.

“I’m currently out of the country, but if what I’ve written here qualifies as an offence under the terms of the new Act, I look forward to being arrested when I return to the birthplace of the Scottish Enlightenment.”

Rowling posted pictures of 10 high-profile trans people on Twitter and mocked their claims to be women. They included Isla Bryson, who was initially sent to a women’s prison after being convicted of two rapes.

wikileaks true value (smear and denial - part 2)

medialens | The UK and US media smears described in Part 1 should be kept in mind when considering the gravity and importance of the latest WikiLeaks. In addition to thousands of previously unreported civilian killings, the leaks revealed more than 1,300 claims of torture by Iraqi police and military between 2005 and 2009. More than 180,000 people were detained at some point between 2004 and 2009, or one in 50 Iraqi males.

But these are only the incidents the US military knew about, or chose to know about, or chose to report; and the documents are an unknown sample of all documentation held by the US government. There are, for example, no reports from the “shock and awe” year of 2003, and none from the tens of thousands of after-attack Pentagon bombing assessments. The leaks also report no civilian deaths in major US atrocities, including the offensive that devastated Fallujah in 2004.

The leaks corroborate previous allegations that US forces turned over prisoners to the Wolf Brigade, the feared 2nd battalion of the Iraqi interior ministry's commandos, infamous for their torture and extra judicial killings. This was not merely ‘turning a blind eye’ to torture, as investigative journalist Gareth Porter notes: “The implication was that the Shi'a commandos would be able to extract more information from the detainees than would be allowed by U.S. rules.”

US forces, then, were complicit in the torture. Indeed, under international law, as the occupying power, the coalition is accountable for all of these crimes.

US troops are actually commanded to not investigate the tortures by an order called Frago 242. Issued in June 2004, this instructs coalition troops not to investigate any abuse of detainees unless it directly involves members of the coalition. Where the alleged abuse is committed by Iraqi forces on Iraqis, "only an initial report will be made... No further investigation will be required unless directed by HQ".
The leaks reveal that the US military was also aware that the Iraqi government had murdered detainees.

Civilian Deaths - The “Standard Accepted Figure”
The leaks reveal, not just a staggering level of violence and criminality in occupied Iraq, but also the determination of the Iraqi government and US forces to hide civilian casualties.

This is hardly surprising and fits with evidence that the US and UK governments have worked hard to smear credible scientific analysis of the likely death toll. A recent study by Professor Brian Rappert of the University of Exeter reported of the UK government: “deliberations were geared in a particular direction – towards finding grounds for rejecting the [2004] Lancet study [estimating almost 100,000 Iraqi deaths from the war] without any evidence of countervailing efforts by government officials to produce or endorse alternative other studies or data”.

Nevertheless, with a near-uniform intellectual sleight of hand, journalists have managed to turn evidence that civilian casualties are likely much higher (as much as ten times higher) than most media have been reporting into evidence that casualties are perhaps 15 per cent higher. As one seasoned journalist told us privately, “WikiLeaks has been Guardianised” - their true significance has been disarmed, defanged and contained by the media.

Might I Owe Wuhan Virological Institute Director Shi Zhengli An Apology?

WashingtonTimes |  Chinese government researchers isolated more than 2,000 new viruses, including deadly bat coronaviruses, and carried out scientific work on them just three miles from a wild animal market identified as the epicenter of the COVID-19 pandemic.

Several Chinese state media outlets in recent months touted the virus research and lionized in particular a key researcher in Wuhan, Tian Junhua, as a leader in bat virus work.

The coronavirus strain now infecting hundreds of thousands of people globally mutated from bats believed to have infected animals and people at a wild animal market in Wuhan. The exact origin of the virus, however, remains a mystery.

Reports of the extensive Chinese research on bat viruses likely will fuel more calls for Beijing to make public what it knows about such work.

“This is one of the worst cover-ups in human history, and now the world is facing a global pandemic,” Rep. Michael T. McCaul, Texas Republican and ranking member of the House Foreign Affairs Committee, said last week. Mr. McCaul has said China should be held accountable for the pandemic.

A video posted online in December and funded by the Chinese government shows Mr. Tian inside caves in Hubei province taking samples from captured bats and storing them in vials.

“I am not a doctor, but I work to cure and save people,” Mr. Tian says in the video. “I am not a soldier, but I work to safeguard an invisible national defense line.”

Fauci Transferred Gain Of Function Research To Wuhan In 2015 After Obama Banned It

newsweek |   The NIH research consisted of two parts. The first part began in 2014 and involved surveillance of bat coronaviruses, and had a budget of $3.7 million. The program funded Shi Zheng-Li, a virologist at the Wuhan lab, and other researchers to investigate and catalogue bat coronaviruses in the wild. This part of the project was completed in 2019.

A second phase of the project, beginning that year, included additional surveillance work but also gain-of-function research for the purpose of understanding how bat coronaviruses could mutate to attack humans. The project was run by EcoHealth Alliance, a non-profit research group, under the direction of President Peter Daszak, an expert on disease ecology. NIH canceled the project just this past Friday, April 24th, Politico reported. Daszak did not immediately respond to Newsweek requests for comment.

The project proposal states: "We will use S protein sequence data, infectious clone technology, in vitro and in vivo infection experiments and analysis of receptor binding to test the hypothesis that % divergence thresholds in S protein sequences predict spillover potential."

In layman's terms, "spillover potential" refers to the ability of a virus to jump from animals to humans, which requires that the virus be able to receptors in the cells of humans. SARS-CoV-2, for instance, is adept at binding to the ACE2 receptor in human lungs and other organs.

According to Richard Ebright, an infectious disease expert at Rutgers University, the project description refers to experiments that would enhance the ability of bat coronavirus to infect human cells and laboratory animals using techniques of genetic engineering. In the wake of the pandemic, that is a noteworthy detail.

Ebright, along with many other scientists, has been a vocal opponent of gain-of-function research because of the risk it presents of creating a pandemic through accidental release from a lab.

 Dr. Fauci is renowned for his work on the HIV/AIDS crisis in the 1990s. Born in Brooklyn, he graduated first in his class from Cornell University Medical College in 1966. As head of NIAID since 1984, he has served as an adviser to every U.S. president since Ronald Reagan.

A decade ago, during a controversy over gain-of-function research on bird-flu viruses, Dr. Fauci played an important role in promoting the work. He argued that the research was worth the risk it entailed because it enables scientists to make preparations, such as investigating possible anti-viral medications, that could be useful if and when a pandemic occurred.

The work in question was a type of gain-of-function research that involved taking wild viruses and passing them through live animals until they mutate into a form that could pose a pandemic threat. Scientists used it to take a virus that was poorly transmitted among humans and make it into one that was highly transmissible—a hallmark of a pandemic virus. This work was done by infecting a series of ferrets, allowing the virus to mutate until a ferret that hadn't been deliberately infected contracted the disease.

 Triple fist tap and elbow bump Dale.

Have 99.999% Missed The Real Revolutionary Possibilities of Crypto?

hackernoon |  Money is power.

Nobody knew this better than the kings of the ancient world. That’s why they gave themselves an absolute monopoly on minting moolah.

They turned shiny metal into coins, paid their soldiers and their soldiers bought things at local stores. 

The king then sent their soldiers to the merchants with a simple message:

“Pay your taxes in this coin or we’ll kill you.”

That’s almost the entire history of money in one paragraph. Coercion and control of the supply with violence, aka the “violence hack.” The one hack to rule them all.

When power passed from monarchs to nation-states, distributing power from one strongman to a small group of strongmen, the power to print money passed to the state. Anyone who tried to create their own money got crushed.

The reason is simple:

Centralized enemies are easy to destroy with a “decapitation attack.” Cut off the head of the snake and that’s the end of anyone who would dare challenge the power of the state and its divine right to create coins.

Kings and nation states know the real golden rule: Control the money and you control the world.

And so it’s gone for thousands and thousands of years. The very first emperor of China, Qin Shi Huang (260–210 BC), abolished all other forms of local currency and introduced a uniform copper coin. That’s been the blueprint ever since. Eradicate alternative coins, create one coin to rule them all and use brutality and blood to keep that power at all costs.

In the end, every system is vulnerable to violence.

Well, almost every one.

Can You Even Imagine The Press Kwestioning Biden Like This?!?!?!

thesaker |   The first Taliban press conference after this weekend’s Saigon moment geopolitical earthquake, conducted by spokesman Zabihullah Mujahid, was in itself a game-changer.

The contrast could not be starker with those rambling pressers at the Taliban embassy in Islamabad after 9/11 and before the start of the American bombing – proving this is an entirely new political animal.

Yet some things never change. English translations remain atrocious.

Here is a good summary of the key Taliban statements, and

here (in Russian) is a very detailed roundup.

These are the key takeaways.

– No problem for women to get education all the way to college, and to continue to work. They just need to wear the hijab (like in Qatar or Iran). No need to wear a burqa. The Taliban insists, “all women’s rights will be guaranteed within the limits of Islamic law.”

– The Islamic Emirate “does not threaten anyone” and will not treat anyone as enemies. Crucially, revenge – an essential plank of the Pashtunwali code – will be abandoned, and that’s unprecedented. There will be a general amnesty – including people who worked for the former NATO-aligned system. Translators, for instance, won’t be harassed, and don’t need to leave the country.

– Security of foreign embassies and international organizations “is a priority.” Taliban special security forces will protect both those leaving Afghanistan and those who remain.

– A strong inclusive Islamic government will be formed. “Inclusive” is code for the participation of women and Shi’ites.

– Foreign media will continue to work undisturbed. The Taliban government will allow public criticism and debate. But “freedom of speech in Afghanistan must be in line with Islamic values.”

– The Islamic Emirate of Taliban wants recognition from the “international community” – code for NATO. The overwhelming majority of Eurasia and the Global South will recognize it anyway. It’s essential to note, for example, the closer integration of the expanding SCO – Iran is about to become a full member, Afghanistan is an observer – with ASEAN: the absolute majority of Asia will not shun the Taliban.

For the record, they also stated that the Taliban took all of Afghanistan in only 11 days: that’s pretty accurate. They stressed “very good relations with Pakistan, Russia and China.” Yet the Taliban don’t have formal allies and are not part of any military-political bloc. They definitely “won’t allow Afghanistan to become a safe haven for international terrorists”. That’s code for ISIS/Daesh.

On the key issue of opium/heroin: the Taliban will ban their production. So, for all practical purposes, the CIA heroin rat line is dead.

As eyebrow raising as these statements may be, the Taliban did not even get into detail on economic/infrastructure development deals – as they will need a lot of new industries, new jobs and improved Eurasian-wide trade relations. That will be announced later.

The go-to Russian guy

Sharp US observers are remarking, half in jest, that the Taliban in only one sitting answered more real questions from US media than POTUS since January.

What this first press conference reveals is how the Taliban are fast absorbing essential P.R. and media lessons from Moscow and Beijing, emphasizing ethnic harmony, the role of women, the role of diplomacy, and deftly defusing in a single move all the hysteria raging across NATOstan.

The next bombshell step in the P.R. wars will be to cut off the lethal, evidence-free Taliban-9/11 connection; afterwards the “terrorist organization” label will disappear, and the Taliban as a political movement will be fully legitimized.

Moscow and Beijing are meticulously stage-managing the Taliban reinsertion in regional and global geopolitics. This means that ultimately the SCO is stage-managing the whole process, applying a consensus reached after a series of ministerial and leaders meetings, leading to a very important summit next month in Dushanbe.

Sen. Rand Paul Goes In On Lil'Fauci's Gain Of Function Culpability

NYMag  |  Take, for instance, this paper from 1995: “High Recombination and Mutation Rates in Mouse Hepatitis Viruses Suggest That Coronaviruses May Be Potentially Important Emerging Viruses.” It was written by Dr. Ralph Baric and his bench scientist, Boyd Yount, at the University of North Carolina. Baric, a gravelly voiced former swim champion, described in this early paper how his lab was able to train a coronavirus, MHV, which causes hepatitis in mice, to jump species, so that it could reliably infect BHK (baby-hamster kidney) cell cultures. They did it using serial passaging: repeatedly dosing a mixed solution of mouse cells and hamster cells with mouse-hepatitis virus, while each time decreasing the number of mouse cells and upping the concentration of hamster cells. At first, predictably, the mouse-hepatitis virus couldn’t do much with the hamster cells, which were left almost free of infection, floating in their world of fetal-calf serum. But by the end of the experiment, after dozens of passages through cell cultures, the virus had mutated: It had mastered the trick of parasitizing an unfamiliar rodent. A scourge of mice was transformed into a scourge of hamsters. And there was more: “It is clear that MHV can rapidly alter its species specificity and infect rats and primates,” Baric said. “The resulting virus variants are associated with demyelinating diseases in these alternative species.” (A demyelinating disease is a disease that damages nerve sheaths.) With steady prodding from laboratory science, along with some rhetorical exaggeration, a lowly mouse ailment was morphed into an emergent threat that might potentially cause nerve damage in primates. That is, nerve damage in us.

"And we need to stop hunting for new exotic diseases in the wild, shipping them back to laboratories, and hot-wiring their genomes to prove how dangerous to human life they might become." https://t.co/N2Cnhk5Xde

— Senator Rand Paul (@RandPaul) May 11, 2021

A few years later, in a further round of “interspecies transfer” experimentation, Baric’s scientists introduced their mouse coronavirus into flasks that held a suspension of African-green-monkey cells, human cells, and pig-testicle cells. Then, in 2002, they announced something even more impressive: They’d found a way to create a full-length infectious clone of the entire mouse-hepatitis genome. Their “infectious construct” replicated itself just like the real thing, they wrote.

Not only that, but they’d figured out how to perform their assembly seamlessly, without any signs of human handiwork. Nobody would know if the virus had been fabricated in a laboratory or grown in nature. Baric called this the “no-see’m method,” and he asserted that it had “broad and largely unappreciated molecular biology applications.” The method was named, he wrote, after a “very small biting insect that is occasionally found on North Carolina beaches.”

In 2006, Baric, Yount, and two other scientists were granted a patent for their invisible method of fabricating a full-length infectious clone using the seamless, no-see’m method. But this time, it wasn’t a clone of the mouse-hepatitis virus — it was a clone of the entire deadly human SARS virus, the one that had emerged from Chinese bats, via civets, in 2002. The Baric Lab came to be known by some scientists as “the Wild Wild West.” In 2007, Baric said that we had entered “the golden age of coronavirus genetics.”

“I would be afraid to look in their freezers,” one virologist told me.

Baric and Shi Zhengli of the Wuhan Institute of Virology, the two top experts on the genetic interplay between bat and human coronaviruses, began collaborating in 2015.